Method for measuring endotoxin
AssigneeSeikagaku Corporation
InventorsHikaru Mizumura, Maki Aizawa, Toshio Oda
Life of this patent
Estimating the term…
Abstract
A method for rapidly and highly sensitively measuring endotoxin relies on an endotoxin-measuring agent, which includes a factor C derived from Tachypleus tridentatus that does not have His-tag sequence at the C-terminus, a factor B of a horseshoe crab, and a proclotting enzyme of a horseshoe crab. Each of these proteins can be a recombinant protein obtainable by being expressed using a stably expressing cell line of an insect cell as a host:
Claims8
An endotoxin-measuring agent comprising the proteins (1) to (3) below, each of which is a recombinant protein obtainable by being expressed using a stably expressing cell line of an insect cell as a host:
- (1) a factor C derived from Tachypleus tridentatus, which factor C does not have His-tag sequence at the C-terminus;
- (2) a factor B of a horseshoe crab;
- and
- (3) a proclotting enzyme of a horseshoe crab.
A factor C derived from Tachypleus tridentatus, which factor C is obtainable by being expressed using a stably expressing cell line of an insect cell as a host, and does not have His-tag sequence at the C-terminus.
Dependencies are inferred from the claim text.