Chromogen layering for color generation
AssigneeDiagnostic BioSystems
InventorsBIPIN GUPTA, MARC KEY
Life of this patent
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Abstract
The present disclosure relates methods of chromogen layering, wherein a first chromogen and a first stain (color) are produced on a sample, specific for a first analyte, followed by a second chromogen and a second stain (color) being produced on the same sample, specific for a second analyte. In addition, if desired, by overlaying the second stain on top of the first stain, a unique third color is produced that is specific for a third analyte. Therefore, the distribution of different colors throughout the sample could be used to identify at least two or more analytes simultaneously within a single sample.
Claims18
A method of staining one section of a biological specimen for one or more different analytes, comprising:
- obtaining a section of a biological specimen;
- contacting the section with a first antibody directed towards a first analyte, wherein the first antibody is labeled with a first marker or wherein the first antibody becomes labeled with a first marker by means of a linking step;
- contacting the section with a first chromogen that reacts with the first marker to generate a first color;
- contacting the section with a second antibody directed towards a second analyte, wherein the second antibody is labeled with a second marker or where the second antibody becomes labeled with a second marker by means of a linking step;
- contacting the section with a second chromogen that reacts with the second marker to generate a second color;
- and
- observing the presence or absence of a first, second, or a third color wherein the third color is a result of overlapping of the first and second colors.
A method of staining one section of a biological specimen for one or more different analytes, comprising:
- obtaining a section of a biological specimen;
- contacting the section with a first probe directed towards a first analyte, wherein the first probe is labeled with a first marker or wherein the first probe becomes labeled with a first marker by means of a linking step;
- contacting the section with a first chromogen that reacts with the first marker to generate a first color;
- contacting the section with a second probe directed towards a second analyte, wherein the second probe is labeled with a second marker or where the second probe becomes labeled with a second marker by means of a linking step;
- contacting the section with a second chromogen that reacts with the second marker to generate a second color;
- and
- observing the presence or absence of a first, second, or a third color wherein the third color is a result of overlapping of the first and second colors.
A method of staining one section of a biological specimen for one or more different analytes, comprising:
- obtaining a section of a biological specimen;
- contacting the section with a first antibody directed towards a first analyte, wherein the first antibody is labeled with a first marker or wherein the first antibody becomes labeled with a first marker by means of a linking step;
- contacting the section with a first chromogen that reacts with the first marker to generate a first color;
- contacting the section with a second chromogen that reacts with the first marker to generate a second color;
- and
- observing the presence or absence of a first and second colors that together form a third color wherein the third color is a result of overlapping of the first and second colors.
Dependencies are inferred from the claim text.