Bioresorbable Exopolysaccharides
AssigneeDePuy Synthes Products, Inc.
InventorDouglas D. Buechter
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Abstract
The present disclosure is directed to microorganisms that are genetically modified to express at least one exogenous enzyme involved in N-acetylglucosamine uptake and metabolism. Methods for the production of an exopolysaccharide are also disclosed. The exopolysaccharide comprises N-acetylglucosamine and D-glucose and may be used as a bioresorbable implant for soft tissue repair, replacement, or augmentation.
Claims40
A microorganism for the production of an exopolysaccharide, wherein:
- a. the microorganism expresses an exogenous enzyme, wherein the exogenous enzyme is N-acetylglucosamine phosphoenolpyruvate-dependent sugar phosphotransferase system permease or a biologically active homologue thereof;
- and
- b. the microorganism produces an exopolysaccharide that comprises N-acetylglucosamine and D-glucose.
A bacterium for the production of an exopolysaccharide, wherein:
- a. the bacterium expresses at least one exogenous enzyme, wherein the at least one exogenous enzyme is N-acetylglucosamine kinase, yeast-derived phosphoacetyl-glucosamine mutase, or UDP-N-acetylglucosamine pyrophosphorylase;
- and
- b. the bacterium produces an exopolysaccharide that comprises N-acetylglucosamine and D-glucose;
- and
- wherein
- the N-acetylglucosamine kinase and the UDP-N-acetylglucosamine pyrophosphorylase are derived from a source other than yeast, or
- the N-acetylglucosamine kinase is derived from a source other than yeast and the UDP-N-acetylglucosamine pyrophosphorylase is derived from yeast, or
- the N-acetylglucosamine kinase is derived from yeast and the UDP-N-acetylglucosamine pyrophosphorylase is derived from a source other than yeast.
A method for producing an exopolysaccharide comprising:
- a. culturing a microorganism under conditions effective to produce the exopolysaccharide, and
- b. recovering the exopolysaccharide,
- wherein said microorganism expresses an exogenous enzyme, wherein the exogenous enzyme is N-acetylglucosamine phosphoenolpyruvate-dependent sugar phosphotransferase system permease or a biologically active homologue thereof, and
- wherein the exopolysaccharide comprises N-acetylglucosamine and D-glucose.
A method for producing an exopolysaccharide comprising:
- a. culturing a bacterium under conditions effective to produce the exopolysaccharide, and
- b. recovering the exopolysaccharide,
- wherein said bacterium expresses at least one exogenous enzyme, wherein the at least one exogenous enzyme is N-acetylglucosamine kinase, yeast-derived phosphoacetyl-glucosamine mutase, or UDP-N-acetylglucosamine pyrophosphorylase, wherein the exopolysaccharide comprises N-acetylglucosamine and D-glucose, and wherein
- the N-acetylglucosamine kinase and the UDP-N-acetylglucosamine pyrophosphorylase are derived from a source other than yeast, or
- the N-acetylglucosamine kinase is derived from a source other than yeast and the UDP-N-acetylglucosamine pyrophosphorylase is derived from yeast, or
- the N-acetylglucosamine kinase is derived from yeast and the UDP-N-acetylglucosamine pyrophosphorylase is derived from a source other than yeast.
Dependencies are inferred from the claim text.