Analysis of single-stranded rna
AssigneeVilnius University
InventorsSaulius KLIMASAUSKAS, Giedrius VILKAITIS, Milda MICKUTE
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Abstract
Provided is a method for modifying a ssRNA at the 3′ end, said method comprising contacting the strand with a ssRNA 2′-O-methyltransferase in the presence of a co-factor, under conditions which allow for the transfer by the ssRNA 2′-O-methyltransferase of a part of the co-factor onto 3′ end of the ssRNA to form a modified ssRNA, wherein the ssRNA bears 2′-OH group at 3′ terminal nucleotide and wherein the part of the co-factor transferred comprises a reporter group or a functional group.
Claims24
1-27. (canceled)
A method for modifying single-stranded RNA (ssRNA) at the 3′ end, said method comprising contacting the strand with a ssRNA 2′-O-methyltransferase in the presence of a co-factor, under conditions which allow for the transfer by the ssRNA 2′-O-methyltransferase of a part of the co-factor onto the 3′ end of the RNA strand to form a modified RNA strand, wherein the ssRNA bears unmethylated 2′-OH group at 3′ terminal nucleotide and wherein the part of the co-factor transferred comprises more than 4 atoms and contains a reporter group or a functional group.
A kit for use in labelling ssRNA, comprising in separate containers (a) a co-factor comprising a reporter group or a functional group;
- and (b) an ssRNA 2′O-methyltransferase capable of transferring the reporter group or the functional group onto the 3′ end of ssRNA wherein the ssRNA bears 2′-OH group at 3′ terminal nucleotide.
Dependencies are inferred from the claim text.