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AssigneePhytowelt Greentechnologies GmbH
InventorsGuido JACH, Sanae AZDOUFFAL, Katrin SCHULLEHNER, Peter WELTERS, Angela NATANEK
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The present invention concerns a method of producing and enantiomerically pure alpha-ionone. Further, the invention concerns a nucleic acid that comprises a sequence that encodes a lycopene-epsilon-cyclase (EC), a lycopene-epsilon-cyclase (EC), plasmids, which encode components of the alpha-ionone biosynthesis and a microorganism that contains heterologous nucleotide sequences which encode the enzymes geranylgeranyl-diphosphate-synthase, isopentenyl-diphosphate-isomerase (IPI), phytoene desaturase-dehydrogenase (crtI), phytoene synthase (crtB), lycopene-epsilon-cyclase (EC) and carotenoid-cleavage-dioxygenase (CCD1). Further, the invention concerns a method of producing highly pure epsilon-carotene.
A method of producing enantiomerically pure alpha-ionone comprising culturing a microorganism that comprises heterologous nucleotide sequences that encode the following enzymes:
A nucleic acid characterized in that it comprises a sequence that encodes a lycopene-epsilon-cyclase (EC) that catalyzes the transformation of lycopene to epsilon-carotene, wherein the encoded lycopene-epsilon-cyclase (EC) leads to a greater epsilon-carotene yield as a reference lycopene-epsilon-cyclase (EC) with a sequence according to SEQ ID NO. 26.
A plasmid characterized in that it comprises nucleotide sequences that encode the following enzymes:
A plasmid characterized in that it comprises nucleotide sequences that encode the following enzymes:
A method of producing highly pure epsilon-carotene from lycopene, comprising culturing a microorganism that contains heterologous nucleotide sequences that encode the following enzymes:
Dependencies are inferred from the claim text.