Time-resolved fluorescent immunochromatographic assay rapid test kit for type i pyrethroid

A time-resolved fluorescent immunochromatographic assay rapid test kit for type I pyrethroid, comprising

• a fluorescent test strip, and a sample reaction vial containing a lyophilized product of an europium-labelled universal monoclonal antibody against type I pyrethroid, wherein the fluorescent test strip comprises a cardboard, the cardboard comprises a plurality of adjacent pads comprising an absorbent pad, a detection pad, and a sample pad adhered in sequence from top to bottom on one side of the cardboard, wherein each of the plurality of adjacent pads are connected at an overlapped junction of the plurality of adjacent pads;
• the detection pad comprises a nitrocellulose membrane as a base pad, a transverse quality control line and a detection line are arranged from top to bottom on the nitrocellulose membrane, the quality control line is coated with rabbit anti-mouse polyclonal antibody, and the detection line is coated with a complete antigen of type I pyrethroid;
• and
• the universal monoclonal antibody against type I pyrethroid is produced from a hybridoma cell line QW8#, deposited on Aug. 30, 2016 to China Center for Type Culture Collection (CCTCC) in Wuhan University, Wuhan, China under CCTCC Accession No. of C2016164.