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US 2018/0237809 A1Published application
Mutant Host Cells For The Production Of 3-Hydroxypropionic Acid
AssigneeNovozymes A/S
InventorsJanice Frias, Guillaume Barbier
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Abstract
Provided herein are recombinant host cells having an active 3-Hydroxypropionic Acid (3-HP) pathway wherein the host cells comprise a disruption to an endogenous gene that encodes for a pyruvate reductase. Also described are methods of making the host cells, and methods using the cells to produce 3-HP and derivatives of 3-HP (e.g., acrylic acid).
Claims20
A recombinant yeast cell, comprising (1) an active 3-HP pathway capable of producing 3-HP and (2) a disruption to an endogenous gene that encodes for a pyruvate reductase, wherein:
- (a) the pyruvate reductase has at least 80% sequence identity to SEQ ID NO:
- 205;
- (b) the coding sequence of the endogenous gene encoding the pyruvate reductase hybridizes under at least high stringency conditions with the full-length complementary strand of SEQ ID NO:
- 204;
- or
- (c) the coding sequence of the endogenous gene encoding the pyruvate reductase has at least 80% sequence identity to SEQ ID NO:
- 204.
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Dependencies are inferred from the claim text.