Compositions and methods for engineering oil content in plants

A method of increasing total oil content or seed yield in a plant or in a tissue, organ, part, cell or propagation material thereof, comprising:

• expressing a transgenic expression cassette in a plant or in a tissue, organ, part, cell or propagation material thereof, wherein the transgenic expression cassette comprises
• a nucleic acid sequence encoding a monoacylglycerol O-acyltransferase 1 (MGAT1) operably linked to a plant-expressible promoter,
• a nucleic acid sequence encoding a phosphatidylcholine diacylglycerol cholinephosphotransferase 1 (PDCT1) operably linked to a plant-expressible promoter,
• a nucleic acid sequence encoding a suppressor of expression of endogenous Sugar Dependent 1 (SPD1) operably linked to a plant-expressible promoter,
• a nucleic acid sequence encoding a diacylglyerol acyltransferase (DGAT1) operatively linked to a plant-expressible promoter,
• a nucleic acid sequence encoding a glycerol-3-phosphate dehydrogenase (GPD1) operatively linked to a plant-expressible promoter
• or a combination thereof;
• and
• selecting the plant or the tissue, organ, part, cell or propagation material thereof in which the total oil content or seed yield of the plant or the total oil content in the tissue, organ, part, cell or propagation material thereof is increased in comparison with a corresponding plant or a tissue organ part, cell or propagation material thereof that is not expressing the transgenic expression cassette.