Hybrid chem-bio method to produce diene molecules
AssigneeTechnology Holding, LLC
InventorsMukund R Karanjikar, Mahesh V Bule, Robert A Price
Life of this patent
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Abstract
A method 100 to produce one or more diene molecules 135 including steps of preparing a biomass hydrolysate 137 from biomass, producing an engineered organism 120 that can feed on the biomass hydrolysate and express an alcohol product useful to make the diene molecule, fermenting 115 the broth with the engineered organism, separating 125 the alcohol product from fermentation broth, and catalyzing 130 the alcohol to create the diene molecule.
Claims20
A method to produce a diene molecule, comprising the steps of:
- providing a carbon-bearing biomass;
- converting the biomass to biomass hydrolysate;
- fermenting the biomass hydrolysate to produce fermentation broth comprising of an alcohol with at least two different reactive sites or two hydroxyl groups;
- separating the alcohol from fermentation broth;
- and
- catalyzing the alcohol to form the diene molecule.
A method to produce an isoprene molecule, comprising the steps of:
- providing a carbon-bearing biomass;
- converting the biomass to a biomass hydrolysate;
- obtaining an engineered organism that can feed on the biomass hydrolysate and subsequently express a desired alcohol product comprising methylbutenol;
- using the engineered organism to ferment the biomass hydrolysate and produce fermentation broth comprising of the desired alcohol product;
- separating the alcohol product from the fermentation broth;
- and
- catalytically converting the alcohol product to form the isoprene molecule.
A method to produce a butadiene molecule, comprising the steps of:
- providing a carbon-bearing biomass;
- converting the biomass to a biomass hydrolysate;
- obtaining an engineered organism that can feed on the biomass hydrolysate and subsequently express a desired alcohol product comprising 2,3-butanediol;
- using the engineered organism to ferment the biomass hydrolysate to and produce fermentation broth comprising of the desired alcohol product;
- separating the alcohol product from fermentation broth;
- and
- catalytically converting the alcohol product to form the butadiene molecule.
Dependencies are inferred from the claim text.