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US 2018/0237321 A1Published application
Engineered yeast as a method for bioremediation
AssigneeMassachusetts Institute of Technology
InventorsGeorge Le-Le Sun, Angela M. Belcher
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Abstract
Metal bioremediation and metal mining strategies can include compositions and methods.
Claims67
A composition for remediating a metal to treat water comprising:
- a cell;
- and
- a first oligomer of a metal binding protein expressed on a surface of the cell via a linker;
- wherein the linker is tethered to the first oligomer of the metal binding protein and to a surface of the cell;
- wherein the metal binding protein has specificity for a metal;
- and
- wherein the first oligomer of the metal binding protein expressed on the surface of the cell is capable of aggregating with a second oligomer of the metal binding protein in the water upon binding a metal.
4 more elements
A method of remediating a metal to treat water comprising:
- preparing a composition comprising:
- a cell;
- and
- a first oligomer of a metal binding protein expressed on a surface of the cell via a linker;
- wherein the linker is tethered to the first oligomer of the metal binding protein and to a surface of the cell;
- wherein the metal binding protein has specificity for a metal;
- and
- wherein the first oligomer of the metal binding protein expressed on the surface of the cell is capable of aggregating with a second oligomer of the metal binding protein in the water upon binding a metal;
- and
- contacting water with the composition.
7 more elements
A method of making a composition for remediating a metal to treat water comprising:
- selecting a cell;
- expressing a metal binding protein on a surface of the cell;
- and
- tethering a first oligomer of a metal binding protein expressed on a surface of the cell via a linker;
- wherein the linker is tethered to the first oligomer of the metal binding protein and to a surface of the cell;
- wherein the metal binding protein has specificity for a metal;
- and
- wherein the first oligomer of the metal binding protein expressed on the surface of the cell is capable of aggregating with a second oligomer of the metal binding protein in the water upon binding a metal.
5 more elements
A method of mining a metal comprising:
- preparing a composition comprising:
- a cell;
- and
- a first oligomer of a metal binding protein expressed on a surface of the cell via a linker;
- wherein the linker is tethered to the first oligomer of the metal binding protein and to a surface of the cell;
- wherein the metal binding protein has specificity for a metal;
- and
- wherein the first oligomer of the metal binding protein expressed on the surface of the cell is capable of aggregating with a second oligomer of the metal binding protein in the water upon binding a metal;
- contacting water with the composition;
- and
- lysing the cell to obtain the metal.
8 more elements
A composition for remediating a metal to treat water comprising:
- a cell expressing
- a membrane metal transporter, wherein the membrane metal transporter has specificity for a metal;
- a vacuole transporter;
- and
- a metal sequestration protein.
2 more elements
A method of remediating a metal to treat water comprising:
- preparing a composition comprising:
- a cell expressing
- a membrane metal transporter, wherein the membrane metal transporter has specificity for a metal;
- a vacuole transporter;
- and
- a metal sequestration protein;
- and
- contacting water with the composition.
5 more elements
A method of mining a metal comprising:
- preparing a composition comprising:
- a cell expressing
- a membrane metal transporter, wherein the membrane metal transporter has specificity for a metal;
- a vacuole transporter;
- and
- a metal sequestration protein;
- contacting water with the composition;
- and
- lysing the cell to obtain the metal.
6 more elements
A composition for remediating a metal to treat water comprising:
- a cell including a knocked-out enzyme required in sulfate-assimilation pathway, wherein the cell has specificity for reactions against a metal.
A method of making a composition for remediating a metal comprising:
- deleting an enzyme required in sulfate-assimilation pathway in a cell, wherein the cell has specificity for reactions against a metal;
- and
- culturing the cell in a medium supplemented with cysteine and/or methionine.
A method of forming a metal nanoparticle comprising:
- preparing a composition comprising a cell including a knocked-out enzyme required in sulfate-assimilation pathway, wherein the cell has specificity for reactions against a metal:
- contacting the composition with water;
- and
- purifying the metal nanoparticle from the cell.
1 more element
A method of remediating a metal to treat water comprising:
- preparing a cell including a knocked-out enzyme required in sulfate-assimilation pathway, wherein the cell has specificity reactions against for a metal;
- and
- contacting water with the composition.
A method of mining a metal comprising:
- preparing a cell including a knocked-out enzyme required in sulfate-assimilation pathway, wherein the cell has specificity reactions against for a metal;
- and
- contacting water with the composition.
Dependencies are inferred from the claim text.