US 2018/0236076 A1 · NEAR INFRARED PHOTOIMMUNOTHERAPY (NIR-PIT) OF SUPPRESSOR CELLS TO TREAT CANCER

Abstract

It is shown that CD25-targeted near-infrared photo-immunotherapy causes a unique, rapid and spatially selective depletion of Tregs leading to regression of the treated tumor and inducing systemic immunologic responses in untreated tumors. Based on these observations, provided are compositions and methods of killing immune suppressor cells, for example to treat cancer. Reducing the number of suppressor cells in a subject can remove suppression of effector T cells, for example, to treat cancer using the subject's own immune system. In particular examples, the method includes contacting suppressor cells having a suppressor cell surface protein with an antibody-IR700 molecule, wherein the antibody specifically binds to the suppressor cell surface protein, and in some examples the antibody does not have a functional Fc region. The cell is subsequently irradiated, such as at a wavelength of 660 to 740 nm, for example at a dose of at least 4 J cm−2.

Claims23

Dependent claims

A method of killing a suppressor cell, comprising:

contacting a suppressor cell comprising a suppressor cell surface protein with a therapeutically effective amount of one or more antibody-IR700 molecules, wherein the antibody specifically binds to the suppressor cell surface protein,
wherein the antibody does not include a functional Fc region;
and/or
wherein the suppressor cell surface protein is one or more of cluster of differentiation 4 (CD4), C-X-C chemokine receptor type 4 (CXCR4), C-C chemokine receptor type 4 (CCR4), cytotoxic T-lymphocyte-associated protein 4 (CTLA4), glucocorticoid induced TNF receptor (GITR), OX40, folate receptor 4 (FR4), CD16, CD56, CD8, CD122, CD23, CD163, CD206, CD11b, Gr-1, CD14, interleukin 4 receptor alpha chain (IL-4Ra), interleukin-1 receptor alpha (IL-1Ra), interleukin-1 decoy receptor, fibroblast activation protein (FAP), CD103, CXCR2, CD33, and CD66b;
and/or
wherein the suppressor cell surface protein comprises CD25 and the antibody does not include a functional Fc region;
irradiating the suppressor cell at a wavelength of 660 to 740 nm and at a dose of at least 4 J cm−2;
thereby killing the suppressor cell.

5 more elements

Dependencies are inferred from the claim text.

Near infrared photoimmunotherapy (nir-pit) of suppressor cells to treat cancer

Life of this patent

Near infrared photoimmunotherapy (nir-pit) of suppressor cells to treat cancer

Life of this patent

Abstract

Claims23