Eimeria tenella elongation factor-1 alpha recombinant immunogenic compositions which induce active protective immunity against avian coccidiosis
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsHYUN S. LILLEHOJ, SUNGTAEK OH, ALFREDO PANEBRA
Life of this patent
Estimating the term…
Abstract
Provided herein are immunogenic compositions containing recombinant proteins capable of presenting all, or antigenic portions of, the Eimeria tenella Elongation Factor 1 alpha, or EF-1α, protein in the development of active immunity to, and control of, coccidiosis. Also provided are methodologies of using the immunogenic compositions for administration to poultry and other animals in the control of coccidiosis. In some instances, the EF-1α protein utilized in the immunogenic composition presented herein is molecularly manipulated or combined with adjuvants to increase effectiveness.
Claims18
An immunogenic composition, comprising a pharmaceutically or veterinarily acceptable carrier and a recombinant protein selected from the group consisting of a recombinant protein of SEQ ID NO:
- 2, a recombinant protein having at least 95% homology to SEQ ID NO:
- 2, and a recombinant protein comprising an antigenic portion of SEQ ID NO:
- 2, wherein said immunogenic composition is capable of inducing an immune response to said protein in a recipient.
An immunogenic composition produced according to the process comprising the steps of:
- a. culturing a recombinant host cell transformed with SEQ ID NO:
- 1, a DNA sequence encoding a protein having at least 95% homology to SEQ ID NO:
- 2, or a DNA sequence encoding a protein comprising an antigenic portion of SEQ ID NO:2;
- b. expressing the protein encoded by the transforming nucleic acid in the recombinant host cell;
- c. purifying the protein produced in the expressing step;
- and
- d. incorporating the purified protein in or on a pharmacologically or veterinarily acceptable carrier.
Dependencies are inferred from the claim text.