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US 2018/0224465 A1Published application
Endothelial Cell Derived Exosomes and Uses Thereof
OwnerEdward Goetzlinventor
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Abstract
The present invention relates to endothelial cell biomarkers and diagnostic and prognostic methods for vascular diseases, including cardiovascular and cerebrovascular diseases. The invention also provides compositions for detecting endothelial cell biomarkers (e.g., endothelial cell-derived exosome biomarkers) as well as compositions and methods useful for treating vascular diseases (e.g., atherosclerotic cerebrovascular disease).
Claims17
A method comprising:
- a) providing a biological sample comprising endothelial-derived exosomes from a subject having a cardiovascular or cerebrovascular disease;
- b) enriching the sample for endothelial-derived exosomes;
- and c) detecting the presence of one or more biomarkers selected from the group consisting of CD81, VCAM-1, eNOS, vWF, PDGF, GPVI, YAP, TAZ, p-selectin, e-selectin, ACE/CD143, C1qR1/CD93, VE-Cadherin, CC Chemokine Receptor D6, Angiopoietin-2 (Tie-2), TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, TRA-1-85/CD147, TRAIL R1/TNFRSF10A, TRAIL R2/TNFRSF10B, VCAM-1/CD106, VE-Statin, VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4, SLAM/CD150, Stabilin-1, Stabilin-2, TEM7/PLXDC1, TEM8/ANTXR1, Thrombomodulin/BDCA-3, THSD1, THSD7A, GLUT-1, CD98/LAT1, NOSTRIN, LOXL2, and p-glycoprotein in the sample.
A method comprising:
- a) providing a biological sample comprising endothelial-derived exosomes from a subject having a cardiovascular or cerebrovascular disease;
- b) isolating endothelial cell-derived exosomes from the biological sample;
- and c) detecting the presence of one or more biomarkers selected from the group consisting of CD81, VCAM-1, eNOS, vWF, PDGF, GPVI, YAP, TAZ, p-selectin, e-selectin, ACE/CD143, C1qR1/CD93, VE-Cadherin, CC Chemokine Receptor D6, Tie-2, TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, TRA-1-85/CD147, TRAIL R1/TNFRSF10A, TRAIL R2/TNFRSF10B, VCAM-1/CD106, VE-Statin, VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4, SLAM/CD150, Stabilin-1, Stabilin-2, TEM7/PLXDC1, TEM8/ANTXR1, Thrombomodulin/BDCA-3, THSD1, THSD7A, GLUT-1, CD98/LAT1, NOSTRIN, LOXL2, and p-glycoprotein in the exosomes.
A method for treating a subject having a cardiovascular or cerebrovascular disease, comprising the steps of:
- providing a biological sample from a subject suspected of having a cardiovascular or cerebrovascular disease, wherein the sample comprises endothelial cell-derived exosomes;
- measuring the level of one or more biomarkers selected from the group consisting of CD81, VCAM-1, eNOS, vWF, PDGF, GPVI, YAP, TAZ, p-selectin, e-selectin, ACE/CD143, C1qR1/CD93, VE-Cadherin, CC Chemokine Receptor D6, Tie-2, TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, TRA-1-85/CD147, TRAIL R1/TNFRSF10A, TRAIL R2/TNFRSF10B, VCAM-1/CD106, VE-Statin, VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4, SLAM/CD150, Stabilin-1, Stabilin-2, TEM7/PLXDC1, TEM8/ANTXR1, Thrombomodulin/BDCA-3, THSD1, THSD7A, GLUT-1, CD98/LAT1, NOSTRIN, LOXL2, and p-glycoprotein from the biological sample, wherein an altered level of the one or more biomarkers in the sample relative to the level in a control sample is indicative of a need for treatment;
- and administering an effective amount of an agent to the subject thereby treating the cardiovascular or cerebrovascular disease in the subject.
Dependencies are inferred from the claim text.