Method for making an epoxy resin mold from a lithography patterned microstructure master

A three dimensional microstructure device for molding a microfluidic device for culturing cells, the three dimensional microstructure comprising:

• epoxy resin;
• a feature less than about 10 μm in height;
• and
• a feature greater than about 100 μm in height.

A method for pattern transfer of a microstructure pattern, the method of microstructure pattern transfer comprising the steps of:

• contacting a silicone-based material with a master template, wherein the master template includes a three dimensional pattern;
• curing the silicone-based substance while in contact with the three dimensional pattern of the master template such that the cured silicone-based material has a three dimensional pattern that substantially corresponds to the three dimensional pattern of the master template;
• removing the cured silicone-based material from the master template;
• contacting an epoxy-resin based material with the three dimensional pattern of the cured silicone-based material;
• curing the epoxy resin-based material while in contact with the three dimensional pattern of the cured silicone-based material such that the cured epoxy-resin based material is a substantial replicate of the three dimensional pattern of the silicone-based material;
• and
• removing the cured epoxy-resin based material from the cured silicone-based material.