Triglyceride oils having asymmetric triglyceride molecules

A method of preparing a triglyceride oil, the triglyceride oil comprising a first population of asymmetric triglyceride molecules and/or a second population of asymmetric triglyceride molecules, the first population comprising triglyceride molecules consisting of a C8:0 fatty acid or a C10:0 fatty acid at the sn-1 position and the sn-2 position, and C14:0, C16:0 or C18:0 at the sn-3 position, the second population comprising triglyceride molecules consisting of a C14:0, C16:0 fatty acid or a C18:0 fatty acid at the sn-1 position and the sn-2 position, and C8:0 or C10:0 fatty acid at the sn-3 position, the method comprising the steps of:

• a. obtaining a triglyceride oil isolated from a recombinant microalgal cell, wherein the recombinant microalgal cell comprises an exogenous gene encoding an active sucrose invertase;
• and
• b. hydrogenating the triglyceride oil to produce the asymmetric triglyceride molecules.