Storage method and banking system of nt cell
AssigneesSungkwang Medical Foundation, Cha Biotech Co., Ltd.
InventorsKwang Yul CHA, Dong Ryul LEE, Young Gie CHUNG, Jihwan SONG, Jin Hee EUM
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Abstract
Provided are a storage method and a banking system of cells prepared using somatic cell nuclear transfer (NT) technology with homozygous genotypes of genes of human leukocyte antigen (HLA)-A, HLA-B, HLA-DR, and the like. The banking of NT cell-derived stem cells may be applied to autologous or allogenic patients and can provide transplantable cells and tissue materials for the treatment of various diseases such as diabetes, osteoarthritis, Parkinson's disease, and the like.
Claims22
A method of storing immunocompatible nuclear transfer (NT) cell-derived stem cells, the method comprising:
- a) screening for homozygosity in a plurality of donor tissues;
- b) isolating nuclei from homozygous cells to generate NT cells;
- c) generating stem cells from the NT cells;
- and
- d) cryopreserving the stem cells.
A method of generating immunocompatible nuclear transfer (NT) cell-derived stem cells, the method comprising:
- a) screening for homozygosity in a plurality of donor tissues;
- b) isolating nuclei from homozygous cells to generate NT cells;
- and
- c) generating stem cells from the NT cells.
A method of generating differentiated cells from immunocompatible NT cell-derived stem cells, the method comprising:
- a) screening for homozygosity in a plurality of donor tissues;
- b) isolating nuclei from homozygous cells to generate NT cells;
- c) generating stem cells from the NT cells;
- and
- d) generating the differentiated cells for transplantation from the stem cells.
A banking system of immunocompatible nuclear transfer (NT) cell-derived stem cells, the banking system comprising:
- a means for collecting a plurality of donor tissues;
- a means for screening for immunocompatibility in the collected donor tissues;
- a means for generating stem cells from immunocompatible tissues, and
- a means for cryopreserving the stem cells.
Dependencies are inferred from the claim text.