Recoverin as a Fusion Protein Tag to Improve Expression, Solubility and Purification of Proteins
AssigneeUniversite Laval
InventorsChristian Salesse, Line Cantin
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Abstract
Herein presented is a new, versatile fusion protein tool (tag) to improve the solubility and purification of proteins. Particularly, the fusion tag is the substantially full-length (about 23 kD) Recoverin molecule (TagR) that is used for protein purification in a single step, even in the presence of detergents.
Claims30
A polynucleotide molecule comprising a sequence encoding a protein of interest to be purified, and a tag polynucleotide encoding a protein of an EF-hand calcium-binding family of proteins, wherein said EF-hand calcium-binding protein undergoes conformational change under presence or absence of calcium wherein the EF-hand calcium-binding protein is a neuronal calcium sensor protein selected from the group consisting of:
- Recoverin, GCAP1, GCAP2, GCAP3, GCIP, KChIP1, KChIP2, Calsenilin/DREAM, NCS1/Frequenin, Neurocalcin delta, Hippocalcin, and VILIP.
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