Microfluidic device

A microfluidic device for separating a phase in a specimen, the microfluidic device comprising a trapping area for capturing the phase of interest, whereby the trapping area is connected on two sides to a first duct and a second duct respectively, both integrated in the microfluidic device, and whereby the microfluidic device further

• comprises a first integrated input connected to the first duct to take the specimen into the trapping area to separate the phase of interest,
• comprises a first integrated output connected to the second duct to discharge the rest of the specimen, once it has flowed through the trapping area,
• has a second integrated output connected to a selected duct selected from the first duct or the second duct, to elute the separated phase out of the device via this output,
• has a second integrated input connected to a first duct or a second duct that is not the selected duct, to connect to a pump to be able to pump the separated phase out of the device, and
• has a third integrated input, also connected to the selected duct via a connection located between the connection of the second integrated output on the selected duct and the microfluidic trapping area and via which the liquid flow during separating of the phase and eluting of the phase may be controlled,
• wherein the third integrated input, when the microfluidic device is in operation and during eluting of the phase of interest, is connected in a circuit to the first integrated input when the third integrated input is in the first duct or to the first integrated output when the third integrated input is in the second duct, thus preventing loss of specimen via the first integrated input or via the first integrated output respectively during eluting.