Back to results
US 2018/0201996 A1Published application
Genotyping by next-generation sequencing
AssigneeData2Bio
InventorsPatrick S. Schnable, Sanzhen Liu, Wei Wu
Life of this patent
Estimating the term…
Abstract
Provided herein is technology relating to genotyping and particularly, but not exclusively, to methods for genotyping one or more organisms by genome sequencing.
Claims25
1-14. (canceled)
A method for genotyping-by-sequencing a sample, the method comprising:
- a) ligating nucleic acid fragments to a first single-stranded oligonucleotide comprising a first primer binding site and a second single-stranded oligonucleotide comprising a second primer binding site, wherein said ligating produces templates comprising single-stranded ends;
- b) amplifying said templates using a first amplification primer that hybridizes to the first primer binding site or complement thereof and a second amplification primer that hybridizes to the second primer binding site or complement thereof to produce amplicons;
- and
- c) genotyping the sample by acquiring sequence reads from the amplicons.
1 more element
A method for genotyping-by-sequencing a sample, the method comprising:
- a) amplifying nucleic acid templates comprising single-stranded ends, said templates produced by ligating nucleic acid fragments to a first single-stranded oligonucleotide comprising a first primer binding site and a second single-stranded oligonucleotide comprising a second primer binding site;
- and
- b) obtaining genotype information by sequencing amplicons produced by said amplifying.
A composition comprising:
- a) a nucleic acid template comprising single-stranded ends, said nucleic acid template comprising:
- i) a restriction fragment;
- ii) a first single-stranded oligonucleotide ligated to the restriction fragment;
- and
- ii) a second single-stranded oligonucleotide ligated to the restriction fragment;
- b) a first primer capable of hybridizing to said first single-stranded oligonucleotide;
- and
- c) a second primer capable of hybridizing to said second single-stranded oligonucleotide.
5 more elements
A method for genotyping-by-sequencing a sample, the method comprising:
- a) ligating nucleic acid fragments to a first nucleic acid consisting essentially of a single-stranded oligonucleotide comprising a first primer binding site and a second nucleic acid consisting essentially of a single-stranded oligonucleotide comprising a second primer binding site, wherein said ligating produces templates comprising single-stranded ends;
- b) amplifying said templates using a first amplification primer that hybridizes to the first primer binding site or complement thereof and a second amplification primer that hybridizes to the second primer binding site or complement thereof to produce amplicons;
- and
- c) genotyping the sample by acquiring sequence reads from the amplicons.
1 more element
Dependencies are inferred from the claim text.