Process for the biological production of methacrylic acid and derivatives thereof
AssigneeLucite International UK Limited
InventorsGraham Ronald EASTHAM, Gill STEPHENS, Andrew YIAKOUMETTI
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Abstract
A process of producing methacrylic acid and/or derivatives thereof including the following steps: (a) biologically converting isobutyryl-CoA into methacrylyl-CoA by the action of an oxidase; and (b) converting methacrylyl-CoA into methacrylic acid and/or derivatives thereof. The invention also extends to microorganisms adapted to conduct the steps of the process.
Claims20
A recombinant microorganism adapted to conduct the following steps:
- (a) biologically converting isobutyryl-CoA into methacrylyl-CoA by expression of an oxidase;
- and
- (b) biologically converting methacrylyl-CoA into methacrylic acid;
- wherein the recombinant microorganism is Escherichia coli.
A microorganism modified by one or more heterologous nucleic acids to conduct the following steps:
- (a) biologically converting isobutyryl-CoA into methacrylyl-CoA by expression of an oxidase;
- and
- (b) biologically converting methacrylyl-CoA into methacrylic acid.
A microorganism adapted to conduct the following steps:
- (a) biologically converting isobutyryl-CoA into methacrylyl-CoA by expression of an oxidase;
- and
- (b) biologically converting methacrylyl-CoA into methacrylic acid and/or derivatives thereof.
A microorganism adapted to conduct the following steps:
- (a) biologically converting isobutyryl-CoA into methacrylyl-CoA by expression of an acyl-CoA oxidase;
- and
- (b) biologically converting methacrylyl-CoA into a C1 to C20 methacrylic acid esterby expression of an alcohol acyl transferase.
A microorganism adapted to conduct the following steps:
- (a) biologically converting isobutyryl-CoA into methacrylyl-CoA;
- and
- (b) biologically converting methacrylyl-CoA into methacrylic acid by the action of a thioesterase, transferase, synthetase, and/or a phosphotransacylase and a short chain fatty acid kinase.
Dependencies are inferred from the claim text.