US 2018/0186830 A1 · COUPLED PEPTIDE CHAIN FOR DISSOLVING POORLY SOLUBLE POLYPEPTIDES AND APPLICATION THEREOF FOR SEPARATION AND PURIFICATION IN LIQUID CHROMATOGRAPHY

Abstract

The invention discloses a coupled peptide chain for dissolving poorly soluble polypeptides and an application thereof for separation and purification in liquid chromatography, belonging to the field of biochemistry. A special linker arm is used to link a hydrophilic polypeptide chain with a poorly soluble polypeptide chain to solve the problem that the poorly soluble polypeptide chains cannot be operated in the liquid chromatography, and optimize the combination of hydrophilic amino acids, and then the poorly soluble polypeptide chain and hydrophilic polypeptide chain are broken by hydrolyzing an ester bond, so that the target peptide chain is directly precipitated, the method has the characteristics of simplicity and high efficiency, and the poorly soluble polypeptide product obtained by the method fully meets the standards required by customers.

Claims6

Dependent claims

A coupled peptide chain for dissolving poorly soluble polypeptides, wherein the peptide chain is as shown in formula (I):

X-Ph-Y—(B)n (I),
wherein Ph represents a phenyl ring substituted at 1 and 4 positions;
X represents a hydroxy group or a C1-C4 monohydroxy alkyl group;
Y represents a carbonyl group or a C2-C4 carbonylalkyl group formed after the dehydration and condensation reaction with (B)n;
(B)n represents a hydrophilic polypeptide chain formed by the condensation of n identical or different hydrophilic amino acids, and n is 1.2 to 2 times the number of amino acids of the poorly soluble polypeptide.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/739,544Filed 3 Feb 2016

Priority

CN 201510466145.531 Jul 2015

Published

5 Jul 2018

Term ends

Not estimatedThe source records don’t support an estimate.

Classification18 codes

C07K 1/16
Extraction; Separation; Purification: by chromatography
B01J 20/292
Sorbents specially adapted for preparative, analytical or investigative chromatography: liquid sorbents
C07K 1/107
General methods for the preparation of peptides , i.e. processes for the organic chemical preparation of peptides or proteins of any length: by…
C07K 2/00
Peptides of undefined number of amino acids

Coupled peptide chain for dissolving poorly soluble polypeptides and application thereof for separation and purification in liquid chromatography

Life of this patent

Coupled peptide chain for dissolving poorly soluble polypeptides and application thereof for separation and purification in liquid chromatography

Life of this patent

Abstract

Claims6