A system for calibrating a multi-port amplifier transmitting traffic signals using a plurality of high power amplifiers connecting to an input hybrid matrix and an output hybrid matrix, all located onboard a spacecraft, the system comprising a plurality of input couplers connecting upstream to the input hybrid matrix, a plurality of complex gain adjusters connecting between the input hybrid matrix and the plurality of high power amplifiers, a plurality of output couplers connecting downstream of the output hybrid matrix, and a calibration apparatus including a calibration unit connecting to the plurality of input and output couplers, and a processing unit connecting to the calibration unit for implementing and running calibration algorithms.