Plants having increased tolerance to herbicides
AssigneeBASF SE
InventorsDoreen Schachtschabel, Birgit Steinhardt, Mihiret Tekeste Sisay, Stefan Tresch, Julia Major, Florian Vogt, Frederick Calo, Jens Lerchl
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Abstract
The present invention provides a plant or plant part comprising a polynucleotide encoding a wildtype or mutant TriA polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to herbicides.
Claims25
A plant, plant part, or plant cell comprising a polynucleotide encoding a wildtype or mutant TriA polypeptide, wherein the expression of said polynucleotide confers to the plant, plant part, or plant cell tolerance to herbicides.
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A method for controlling weeds at a locus for growth of a plant, the method comprising:
- (a) applying an herbicide composition comprising herbicides to the locus;
- and (b) planting a seed at the locus, wherein the seed is capable of producing a plant that comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated TriA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated TriA polypeptide conferring to the plant tolerance to herbicides.
A method of producing a plant having tolerance to herbicide, the method comprising regenerating a plant from a plant cell transformed with a recombinant polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated TriA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated TriA polypeptide conferring to the plant tolerance to herbicides.
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An isolated and/or recombinantly produced and/or synthetic nucleic acid molecule comprising a nucleic acid molecule encoding a mutated TriA polypeptide selected from the group consisting of:
- (a) a nucleic acid molecule encoding a mutated TriA polypeptide comprising the sequence of SEQ ID NO:
- 2, or a variant, paralogue, orthologue or homolog thereof;
- (b) a nucleic acid molecule comprising the sequence of SEQ ID NO:
- 1, or a variant, paralogue, orthologue or homolog thereof;
- (c) a nucleic acid molecule, which, as a result of the degeneracy of the genetic code, can be derived from a TriA polypeptide sequence of SEQ ID NO:
- 2, or a variant, paralogue, orthologue or homolog thereof, and confers increased herbicide tolerance or resistance, as compared to a corresponding, e.g. non-transformed, wild type plant cell, a plant or a part thereof;
- (d) a nucleic acid molecule having 30% or more identity with the nucleic acid molecule sequence of a polynucleotide comprising the nucleic acid molecule of SEQ ID NO:
- 1, or a variant, paralogue, orthologue or homolog-thereof, and confers increased herbicide tolerance or resistance, as compared to a corresponding, non-transformed, wild type plant cell, a plant or a part thereof;
- (e) a nucleic acid molecule encoding a mutated TriA polypeptide having 30% or more identity with the amino acid sequence of the TriA polypeptide sequence of SEQ ID NO:
- 2, and confers increased herbicide tolerance or resistance, as compared to a corresponding, non-transformed, wild type plant cell, a plant or a part thereof;
- (f) nucleic acid molecule which hybridizes with a nucleic acid molecule of (a), (b), (c), (d) or (e) under stringent hybridization conditions and confers increased herbicide tolerance or resistance, as compared to a corresponding, non-transformed, wild type plant cell, a plant or a part thereof;
- wherein the amino acid sequence of the mutated TriA polypeptide differs from the wildtype amino acid sequence of a TriA polypeptide at one or more positions corresponding to the following positions of SEQ ID NO:2:
- 70, 71, 88, 91, 92, 96, 126, 128, 155, 157, 167, 220.
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Dependencies are inferred from the claim text.