Protein having synthetic activity for imidazole dipeptid and production method of imidazole dipeptide
AssigneeTokai Bussan Co., Ltd.
InventorKuniki Kino
Life of this patent
Estimating the term…
Abstract
The present invention provides L-amino acid α-ligase represented by sequence number 1 (SEQ ID NO. 1), which is a mutant protein including substitution of at least 1-3 amino acid residues of amino acid sequence of protein YwfE, with the substitution being at least one of an asparagine (N) residue of the 108th place from the N terminal being substituted with at least one of an alanine (A) residue, a glutamic acid (E) residue and a glutamine (Q) residue, an isoleucine (I) residue of the 112th place from the N terminal being substituted with a valine (V) residue, and a histidine (H) residue of the 378th place from the N terminal being substituted with at least one of a lysine (K) residue or an arginine (R) residue.
Claims18
A mutant protein of L-amino acid α-ligase represented by SEQ ID NO:
- 1, the mutant protein including substitution of at least 1-3 amino acid residues of amino acid sequence of protein YwfE, with the substitution being at least one of
- an asparagine (N) residue of a 108th place from an N terminal being substituted with at least one of an alanine (A) residue, a glutamic acid (E) residue and a glutamine (Q) residue,
- an isoleucine (I) residue of a 112th place from the N terminal being substituted with a valine (V) residue, and
- a histidine (H) residue of a 378th place from the N terminal being substituted with at least one of a lysine (K) residue and an arginine (R) residue.
Dependencies are inferred from the claim text.