US 2018/0177892 A1 · COMPOSITIONS OF NUCLEIC ACID-CONTAINING NANOPARTICLES FOR IN VIVO DELIVERY

Abstract

Compositions comprising a polymeric micellar nanoparticle composition comprising a block or graft copolymer comprising at least one polycationic polymer and at least one polyethylene glycol (PEG) polymer having an average molecular weight less than 1 kDa, and at least one nucleic acid, wherein the graft or block copolymer and at least one nucleic acid are complexed and condensed into a shaped micellar nanoparticle that is stable in biological media are disclosed. The presently disclosed subject matter also provides a method for preparing the presently disclosed polymeric micellar nanoparticle compositions, a method for targeting at least one metastatic cancer cell in a subject, and a method for treating a disease or condition using the presently disclosed polymeric micellar nanoparticle compositions.

Claims25

Dependent claims

A polymeric micellar nanoparticle composition, comprising:

(a) a block or graft copolymer comprising at least one polycationic polymer and at least one polyethylene glycol (PEG) polymer having an average molecular weight less than 1 kDa;
and
(b) at least one nucleic acid;
wherein the graft or block copolymer and the at least one nucleic acid are complexed and condensed into a shaped micellar nanoparticle that is stable in biological media.

1 more element

A method for preparing a polymeric micellar nanoparticle composition comprising a block or graft copolymer comprising at least one polycationic polymer and at least one polyethylene glycol (PEG) polymer having an average molecular weight less than 1 kDa;

and at least one nucleic acid;
wherein the graft or block copolymer and the at least one nucleic acid are complexed and condensed into a shaped micellar nanoparticle that is stable in biological media, the method comprising:
(a) mixing a first solution comprising the block or graft copolymer together with a second solution comprising the at least one nucleic acid to form a third solution comprising the block or graft copolymer and the at least one nucleic acid;
and
(b) allowing the block or graft copolymer and the at least one nucleic acid to self-assemble into the polymeric micellar nanoparticle.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/890,771Filed 7 Feb 2018

Priority

14 May 2015

Published

28 Jun 2018

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

A61K 48/0041
Wherein the non-active part clearly interacts with the delivered nucleic acid: the non-active part being polymeric
A61K 45/06
Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00: mixtures of active ingredients without…
A61K 47/59
The modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule: obtained otherwise than by…
A61K 47/60
Obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes: the organic…

Family

Application62/161,546Us provisional application · filed 14 May 2015

Application15/154,143Continuation · filed 13 May 2016

Application15/890,771Continuation

US 11,235,071 B2Related patent

US 2016/0331845 A1Published application

US 2018/0177892 A1This patent

From the US filing history. Foreign family members aren’t listed.

Compositions of nucleic acid-containing nanoparticles for in vivo delivery

Life of this patent

Compositions of nucleic acid-containing nanoparticles for in vivo delivery

Life of this patent

Abstract

Claims25