Alkb -facilitated rna methylation sequencing (arm-seq)
AssigneeThe Regents of the University of California
InventorsTodd LOWE, Aaron COZEN, Eva H. ROBINSON, Andrew D. HOLMES, Eric PHIZICKY, Erin QUARTLEY
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Abstract
In various embodiments, the invention teaches methods for detecting ribonucleic acid (RNA) molecules that contain certain chemical modifications using sequencing technologies. These modified RNAs are otherwise not readily detected using the commonly used cloning protocols required for sequencing. The method further includes bioinformatics analyses to identify specific RNA species that are modified at high resolution.
Claims17
A method, comprising
- providing a ribonucleic acid (RNA);
- and
- applying a quantity of a de-alkylating enzyme to the RNA.
A composition, comprising a ribonucleic acid (RNA) that has been treated with a de-alkylating enzyme.
A kit, comprising:
- a de-alkylating enzyme;
- and
- instructions for the use thereof to sequence an RNA.
Dependencies are inferred from the claim text.