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US 2018/0171362 A1Published application
Assays for evaluating cell culture reagents
AssigneeBiogen MA Inc.
InventorsJessica Mondia, Alan Gilbert, Maureen Lanan
Life of this patent
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Abstract
Some aspects of the present disclosure provide methods, compositions and kits for identifying one or more reagents, such as a culture medium, that are effective for recombinant protein production.
Claims28
A method of promoting recombinant protein yield from a recombinant cell culture in a bioreactor, the method comprising
- performing an assay to determine a level of an inhibitor of proprotein convertase activity in one or more cell culture reagents, and
- using a cell culture reagent to support growth of the recombinant cell culture in the bioreactor only if the cell culture reagent is determined to contain a proprotein convertase inhibitor in an amount that is acceptable for the recombinant protein yield.
A method of selecting a reagent for use in the production of a recombinant protein, the method comprising;
- performing a proprotein convertase substrate assay in the presence of a sample of a reagent;
- determining a level of activity of a proprotein convertase;
- and
- identifying the reagent as acceptable for use in a recombinant cell culture to produce the recombinant protein if the level of activity of the proprotein convertase is at or above a threshold level sufficient for recombinant protein production.
1 more element
Dependencies are inferred from the claim text.