Apparatus for microcarrier filtration and separation from cells and media
AssigneeCorning Incorporated
InventorWilliam Joseph Lacey
Life of this patent
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Abstract
A flow-through apparatus is provided. The flow-through apparatus includes a housing having a fixed volume and having an inlet, an outlet, and a cavity. The flow-through apparatus also includes a first filter in fluid communication with the inlet and disposed within the cavity and a seal attaching the first filter to the housing. The first filter has a mesh size of about 100 microns or less and a first internal volume less than the fixed volume of the housing.
Claims24
A flow-through apparatus comprising:
- a housing having a fixed volume and comprising an inlet, an outlet, and a cavity;
- a first filter in fluid communication with the inlet and disposed within the cavity;
- and
- a seal attaching the first filter to the housing;
- wherein the first filter has a mesh size of about 100 microns or less and a first internal volume less than the fixed volume of the housing.
A flow-through apparatus comprising:
- a flexible bag comprising first and second sidewalls sealed along a periphery to define a cavity comprising an inlet and an outlet;
- and
- a filter disposed within the cavity and having a mesh size of about 100 microns or less;
- wherein the filter is attached to (a) an inside surface of the first sidewall at a first interface located along a length of the bag and (b) an inside surface of the second sidewall at a second interface located along the length of the bag;
- and
- wherein the length of the bag extends from the inlet to the outlet and the first interface is nearer to the inlet than the second interface.
A method for filtering a medium, the method comprising:
- introducing a medium into a flow-through apparatus comprising:
- a housing having a fixed volume and comprising an inlet, an outlet, and a cavity;
- a first filter in fluid communication with the inlet and disposed within the cavity, wherein the first filter has a mesh size of about 100 microns or less and a first internal volume less than the fixed volume of the housing;
- and
- a seal attaching the first filter to the housing;
- wherein the medium enters the inlet and flows into the cavity through the first filter to produce a filtrate and the filtrate exits the cavity through the outlet.
A method for filtering a medium, the method comprising:
- introducing a medium into a flow-through apparatus comprising:
- a flexible bag comprising first and second sidewalls sealed along a periphery to define a cavity comprising an inlet and an outlet;
- and
- a filter disposed within the cavity and having a mesh size of about 100 microns or less;
- wherein the filter is attached to (a) an inside surface of the first sidewall at a first interface located along a length of the bag and (b) an inside surface of the second sidewall at a second interface located along the length of the bag;
- wherein the length of the bag extends from the inlet to the outlet and the first interface is nearer to the inlet than the second interface;
- and
- wherein the medium enters the inlet and flows through the first filter to produce a filtrate and the filtrate exits through the outlet.
Dependencies are inferred from the claim text.