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US 2018/0163242 A1Published application
Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis
AssigneeDanisco US Inc.
InventorsCOLIN MITCHINSON, MEREDITH K. FUJDALA, WILLIAM D. HITZ, MEGAN Y. HSI, STEVEN S. KIM, KEITH D. WING
Life of this patent
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Abstract
The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having glycosyl hydrolase (GH) family 61/endoglucanase activity and/or a β-glucosidase polypeptide, methods for hydrolyzing biomass material, and methods for using such compositions.
Claims49
An engineered enzyme composition, comprising:
- a) a polypeptide having xylanase activity;
- and
- b) a polypeptide having β-xylosidase activity selected from a Group 1 or 2 β-xylosidase;
- and
- c) a polypeptide having L-α-arabinofuranosidase activity;
- and
- d) a polypeptide having β-glucosidase activity or a whole cellulase enriched with the polypeptide having β-glucosidase activity,
- wherein the enzyme composition is capable of hydrolyzing a lignocellulosic biomass material.
5 more elements
An engineered enzyme composition comprising:
- a) a polypeptide having β-xylosidase activity selected from a Group 1 β-xylosidase;
- and
- b) a polypeptide having β-xylosidase activity selected from a Group 2 β-xylosidase;
- and
- c) a polypeptide having L-α-arabinofuranosidase activity;
- and
- d) a polypeptide having β-glucosidase activity or a whole cellulase enriched with the polypeptide having β-glucosidase activity,
- wherein the enzyme composition is capable of hydrolyzing a lignocellulosic biomass material.
5 more elements
An engineered enzyme composition comprising:
- a) a polypeptide having xylanase activity;
- and
- b) a polypeptide having β-xylosidase activity selected from a Group 1 β-xylosidase;
- and
- c) a polypeptide having β-xylosidase activity selected from a Group 2 β-xylosidase;
- and
- d) a polypeptide having β-glucosidase activity or a whole cellulase enriched with the polypeptide having β-glucosidase activity,
- wherein the enzyme composition is capable of hydrolyzing a lignocellulosic biomass material.
5 more elements
An engineered enzyme composition comprising:
- a) a polypeptide having xylanase activity;
- and
- b) a polypeptide having β-xylosidase activity selected from a Group 1 or 2 β-xylosidase;
- and
- c) a polypeptide having β-glucosidase activity or a whole cellulase enriched with the polypeptide having β-glucosidase activity,
- wherein the enzyme composition is capable of hydrolyzing a lignocellulosic biomass material.
3 more elements
An engineered enzyme composition, comprising:
- a) a polypeptide having xylanase activity;
- and
- b) a polypeptide having β-xylosidase activity selected from a Group 1 or 2 β-xylosidase;
- and
- c) a polypeptide having L-α-arabinofuranosidase activity;
- and
- d) a polypeptide having GH61/endoglucanase activity or a whole cellulase enriched with the polypeptide having GH61/endoglucanase activity,
- wherein the enzyme composition is capable of hydrolyzing a lignocellulosic biomass material.
5 more elements
An engineered enzyme composition comprising:
- a) a polypeptide having β-xylosidase activity selected from a Group 1 β-xylosidase;
- and
- b) a polypeptide having β-xylosidase activity selected from a Group 2 β-xylosidase;
- and
- c) a polypeptide having L-α-arabinofuranosidase activity;
- and
- d) a polypeptide having GH61/endoglucanase activity or a whole cellulase enriched with the polypeptide having GH61/endoglucanase activity,
- wherein the enzyme composition is capable of hydrolyzing a lignocellulosic biomass material.
5 more elements
An engineered enzyme composition comprising:
- a) a polypeptide having xylanase activity;
- and
- b) a polypeptide having β-xylosidase activity selected from a Group 1 β-xylosidase;
- and
- c) a polypeptide having β-xylosidase activity selected from a Group 2 β-xylosidase;
- and
- d) a polypeptide having GH61/endoglucanase activity or a whole cellulase enriched with the polypeptide having GH61/endoglucanase activity,
- wherein the enzyme composition is capable of hydrolyzing a lignocellulosic biomass material.
5 more elements
An engineered enzyme composition comprising:
- a) a polypeptide having xylanase activity;
- and
- b) a polypeptide having β-xylosidase activity selected from a Group 1 or 2 β-xylosidase;
- and
- c) a polypeptide having GH61/endoglucanase activity or a whole cellulase enriched with the polypeptide having GH61/endoglucanase activity,
- wherein the enzyme composition is capable of hydrolyzing a lignocellulosic biomass material.
3 more elements
Dependencies are inferred from the claim text.