Process for preparing (1→6)-α-d-glucan
AssigneeSouth China Botanical Garden, Chinese Academy of Sciences
InventorsBao Yang, Yueming Jiang
Life of this patent
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Abstract
A process for preparing (1→6)-α-D-glucan. A large amount of (1→6)-α-D-glucan can be prepared from banana by using this process. The chemical structure of (1→46)-α-D-glucan is shown as Scheme 1. The yield of (1→6)-α-D-glucan is 3-16 g/kg (with a purity of 85%-95%). This process provides a feasible technique for extensive utilization of banana, and can improve the additional value of banana products. This process is helpful for the sustainable development of banana processing industry.
Claims10
A process for preparing (1→6)-α-D-glucan, comprising the following steps:
- extracting banana pulp with water to give a supernatant;
- separating, collecting and concentrating the supernatant,
- adding ethanol into the concentrated supernatant to precipitate crude banana polysaccharides, and collecting the crude banana polysaccharides;
- purifying the crude banana polysaccharides with anion exchange resin, and eluting with water or phosphate solution to obtain a fraction eluted with water or a fraction eluted with phosphate solution;
- concentrating and drying the fraction eluted with water to obtain the (1→6)-α-D-glucan, or, concentrating, dialyzing and drying the fraction eluted with phosphate solution to obtain the (1→6)-α-D-glucan.
A method of using banana pulp in preparing (1→6)-α-D-glucan, comprising the steps:
- extracting banana pulp with water to give a supernatant;
- separating, collecting and concentrating the supernatant,
- adding ethanol into the concentrated supernatant to precipitate crude banana polysaccharides, and collecting the crude banana polysaccharides;
- purifying the crude banana polysaccharides with anion exchange resin, and eluting to obtain a fraction elute;
- concentrating and drying the fraction elute to obtain the (1→6)-α-D-glucan.
Dependencies are inferred from the claim text.