Cahuitamycins and methods of making and using the same
AssigneeThe Regents of the University of Michigan
InventorsDavid H. Sherman, Fengan Yu, Chuanwu Xi, Jianfeng Wu, Pamela Schultz, Ashootosh Tripathi, Sung Ryeol Park
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Abstract
Provided herein are biofilm inhibitors obtained from marine microbial derived natural product extracts from Streptomyces gandocaensis, resulting in biofilm inhibitors, cahuitamycins. Also provided are mutant S. gandocaensis, methods of inhibiting biofilm formation, methods of producing, or increasing the production of, cahuitamycins, methods for synthesizing cahuitamycins, and methods of purifying cahuitamycins.
Claims33
A compound having a structure:
- or a pharmaceutically acceptable salt thereof.
A composition comprising (a) a cahuitamycin compound or pharmaceutically acceptable salt thereof and (b) a pharmaceutically acceptable carrier, wherein the cahuitamycin compound has a structure selected from:
A mutant Streptomyces gandocaensis lacking salicylate synthase activity.
A method of producing a cahuitamycin compound comprising
- (a) culturing Streptomyces gandocaensis in the presence of streptomycin to select for a mutated ribosomal protein, thereby generating a mutated S. gandocaensis;
- and
- (b) obtaining a cahuitamycin compound from the culture of mutated S. gandocaensis.
A method of producing a cahuitamycin compound comprising
- (a) culturing Streptomyces gandocaensis under conditions wherein the activity of salicylate synthase is inhibited;
- and
- (b) obtaining the cahuitamycin compound from the culture.
A method of increasing the production of cahuitamycin A, cahuitamycin B, or a mixture of cahuitamycin A and B comprising
- (a) culturing Streptomyces gandocaensis under conditions wherein the activity of 6-methylsalicylic acid synthase is inhibited;
- and
- (b) obtaining the cahuitamycin compound from the culture.
A method comprising subjecting an extract from Streptomyces gandocaensis to extraction, chromatography, or both to isolate a compound selected from the group consisting of
Dependencies are inferred from the claim text.