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AssigneeGlaxosmithkline Biologicals SA
InventorsFrancesco BERTI, Paolo COSTANTINO, Maria Rosaria ROMANO
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The invention provides a process for preparing a conjugate of a S.aureus type 5 or type 8 capsular polysaccharide and a carrier molecule, comprising the steps of: (a) depolymerising the capsular polysaccharide, to give a polysaccharide fragment; (b) oxidising the fragment in order to introduce an aldehyde group into at least one saccharide residue in the fragment, to give an oxidised saccharide residue; and (c) coupling the oxidised saccharide residue to a carrier molecule via the aldehyde group, thereby giving the conjugate. The coupling in step (c) may be direct, or may be via a linker molecule. The invention also provides a conjugate obtained or obtainable by this process.
A process for preparing a conjugate of a S.aureus type 5 or type 8 capsular polysaccharide and a carrier molecule, comprising the steps of:
A process for treating a S.aureus type 5 capsular polysaccharide comprising the step of depolymerising the capsular polysaccharide, to give a polysaccharide fragment having a β-D-FucNAc-(1→moiety at its non-reducing terminus.
A process for treating a S.aureus type 8 capsular polysaccharide comprising the step of depolymerising the capsular polysaccharide, to give a polysaccharide fragment having an α-D-FucNAc-(1→moiety at is non-reducing terminus.
The process according to any preceding claim, wherein the depolymerising is carried out by acid hydrolysis using acetic acid.
The process according to any preceding claim, wherein the average molecular mass of the fragment is between 5 and 100 kDa.
The process according to any preceding claim, wherein the degree of O-acetylation of the fragment is 10-90%.
A process for providing a S.aureus type 5 capsular polysaccharide derivative comprising the step of oxidising a S.aureus type 5 capsular polysaccharide having a β-D-FucNAc-(1→moiety at its non-reducing terminus to convert two vicinal hydroxyl groups in the β-D-FucNAc-(1→moiety into two aldehyde groups.
A process for providing a S.aureus type 8 capsular polysaccharide derivative comprising the step of oxidising a S.aureus type 8 capsular polysaccharide having an α-D-FucNAc-(1→moiety at is non-reducing terminus to convert two vicinal hydroxyl groups in the α-D-FucNAc-(1→moiety into two aldehyde groups.
A process for providing a coupled S.aureus type 5 capsular polysaccharide comprising the step of coupling to a carrier molecule a S.aureus type 5 capsular polysaccharide having a β-D-FucNAc-1→moiety at its non-reducing terminus that has been oxidised to convert two vicinal hydroxyl groups into two aldehyde groups, wherein the coupling is via one of the aldehyde groups.
A provides a process for providing a coupled S.aureus type 8 capsular polysaccharide comprising the step of coupling to a carrier molecule a S.aureus type 8 capsular polysaccharide having an α-D-FucNAc-(1→moiety at its non-reducing terminus that has been oxidised to convert two vicinal hydroxyl groups into two aldehyde groups, wherein the coupling is via one of the aldehyde groups.
A conjugate, fragment, derivative or coupled polysaccharide obtained or obtainable by the process of any preceding claim.
Dependencies are inferred from the claim text.