Back to results
US 2018/0149651 A1Published application
Proteome Editing System and A Biomarker of Veev Infection
AssigneeThe Government of the United States of America, as represented by the Secretary of the Navy
InventorsPatricia M. Legler, Elaine Morazzani, Pamela Glass, Jaimee R. Compton
Life of this patent
Estimating the term…
Abstract
A protease of the Venezuelan equine encephalitis virus (VEEV) was found to act on a host substrate in addition to the viral substrate. It is contemplated that these findings could be employed to facilitate post-translational silencing at the level of protein (removal of existing proteins) as a protein analog to CRISPR/Cas9 and RNAi/RISC, and further to enable detection of viral infection.
Claims9
A method of detecting infection, comprising:
- obtaining biological material from an individual suspected of being infected with a Group IV virus;
- and
- assaying the biological material to detect the presence or absence of a cleavage product of a protease of the Group IV virus,
- wherein the presence of the cleavage product indicates that the individual is likely infected with the Group IV virus.
1 more element
A method of cleaving a protein, comprising:
- causing a cell to express a recombinant viral polyprotein of a Group IV virus that incorporates a cleavage site recognized by a protease;
- and
- infecting the cell with the Group IV virus, thereby causing the viral protease to cleave the recombinant protein at the cleavage site.
Dependencies are inferred from the claim text.