Enzymatic transphosphorylation of sugar substrates
AssigneeBASF SE
InventorsVaidotas NAVICKAS, Kai-Uwe BALDENIUS, Michael BREUER, Patricia WILDBERGER, Martin PFEIFFER, Bernd NIDETZKY
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods of producing sugar phosphates by enzymatic transphosphorylation, wherein the phosphoryl transfer from phosphate donor substrates to sugar substrates is catalyzed by an enzyme having alpha-glucose-1-phosphatase activity. Furthermore, the present invention relates to a biocatalyst having sucrose phosphorylase as well as alpha-glucose-1-phosphatase activity and its use. The production of an enzyme having alpha-glucose-1-phosphatase activity and its use in the transphosphorylation of sugar substrates is also presented herein.
Claims10
-9. (canceled)
A method of producing one or more sugar phosphates by enzymatic transphosphorylation comprising:
- (i) providing one or more sugar substrates selected from the group consisting of aldoses and ketoses,
- (ii) providing one or more phosphate donor substrates selected from the group consisting of phosphorylated carbohydrates,
- (iii) providing an alpha-glucose-1-phosphatase or an enzyme having alpha-glucose-1-phosphatase activity,
- (iv) incubating the one or more sugar substrates provided in step (i) and the one or more phosphate donor substrates provided in step (ii) with the alpha-glucose-1-phosphatase or, respectively, the enzyme having alpha-glucose-1-phosphatase activity provided in step (iii) under conditions which allow transphosphorylation to obtain one or more sugar phosphates,
- (v) optionally, purifying the one or more sugar phosphates obtained in step (iv).
Dependencies are inferred from the claim text.