Biological methods for preparing 3-hydroxypropionic acid
AssigneeVerdezyne, Inc.
InventorEric Michael KNIGHT
Life of this patent
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Abstract
This technology relates in part to biological methods for producing 3-hydroxypropionic acid and engineered microorganisms capable of such production.
Claims30
A genetically modified yeast, comprising (a) one or more genetic modifications that reduce or abolish the activity of 3-hydroxypropionate dehydrogenase (HPD1) or malonate semialdehyde dehydrogenase (acetylating) (ALD6), and (b) one or more genetic modifications that increases the activity of one or more enzymes selected from among a cytochrome P-450 monooxygenase, a cytochrome P-450 reductase, an aldehyde dehydrogenase, an alcohol dehydrogenase, an acyl-CoA transferase, a long-chain-fatty-acid CoA ligase, an acyl-CoA synthetase, an acetyl-CoA C-acyltransferase, a propionyl-CoA synthetase, an acyl-CoA oxidase, an acyl-CoA dehydrogenase, an enoyl-CoA hydratase, and 3-hydroxypropionyl-CoA hydrolase.
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An expression vector, comprising one or more genetic modifications that reduce or abolish the activity of 3-hydroxypropionate dehydrogenase (HPD1) or malonate semialdehyde dehydrogenase (acetylating) (ALD6).
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A method for producing 3-hydroxypropionic acid, 3-hydroxypropionate or a salt thereof, comprising:
- (a) contacting a genetically modified yeast that comprises one or more genetic modifications that reduce or abolish the activity of 3-hydroxypropionate dehydrogenase (HPD1) or malonate semialdehyde dehydrogenase (acetylating) (ALD6) with a feedstock;
- and (b) culturing the genetically modified yeast under a condition in which the 3-hydroxypropionic acid, 3-hydroxypropionate or salt thereof is produced.
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Dependencies are inferred from the claim text.