Biosynthesis of Phenylpropanoid and Dihydrophenylpropanoid Derivatives
AssigneeEvolva SA
InventorsMichael NAESBY, Ernesto SIMÓN VECILLA, Michael EICHENBERGER, Beata Joanna LEHKA, Walden Emil BJØRN-YOSHIMOTO, Niels Bjerg JENSEN, Jane Dannow DYEKJÆR
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Abstract
Provided herein are methods and compositions for producing phenylpropanoid derivatives, such as chalcones and stilbenes, and dihydrophenylpropanoid derivatives, such as dihydrochalcones and dihydrostilbenes, in microorganisms. In particular, the disclosure provides recombinant microorganisms and methods of use thereof for producing phenylpropanoid derivative compounds and dihydrophenylpropanoid derivative compounds.
Claims86
A method of modulating production of a phenylpropanoid derivative compound relative to a dihydrophenylpropanoid derivative compound in a recombinant host cell, the method comprising:
- (a) increasing production of the phenylpropanoid derivative compound relative to the dihydrophenylpropanoid derivative compound by reducing or eliminating (i) double-bond reductase activity, or (ii) expression of a gene encoding a double-bond reductase polypeptide;
- or
- (b) decreasing production of the phenylpropanoid derivative compound relative to the dihydrophenylpropanoid derivative compound by increasing (i) double-bond reductase activity, or (ii) expression of a gene encoding a double-bond reductase polypeptide;
- wherein the phenylpropanoid derivative compound is a chalcone or stilbene, and wherein the dihydrophenylpropanoid derivative compound is a dihydrochalcone or dihydrostilbene.
A recombinant yeast cell capable of producing a phenylpropanoid or phenylpropanoid derivative compound comprising a gene encoding a double-bond reductase polypeptide, wherein expression of the gene or activity of the double-bond reductase polypeptide encoded thereby is reduced or eliminated.
A recombinant host cell comprising:
- (a) a recombinant gene encoding an enoyl reductase polypeptide;
- and
- (b) a recombinant gene encoding a polyketide synthase Type III polypeptide.
Dependencies are inferred from the claim text.