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US 2018/0135089 A1Published application
Method for Do Novo Biosynthesis of Chondroitin Sulfate
AssigneeJiangnan University
InventorsZhen Kang, Jian Chen, Guocheng Du, Zhengxiong Zhou
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Abstract
The present invention relates to a method for chondroitin sulfate biosynthesis, belongs to the field of pharmaceuticals field. CS was biosynthesized by sulfating the chondroitin with C4ST or C6ST in Tris-HCI buffer assisted with 3′-phosphoadenosine 5′-phophosulfate (PAPS). C4ST and C46ST came from bioengineered Escherichia coli or Pichia pastoris. Chondroitin came from bioengineered Bacillus subtilis 168.
Claims15
A method for biosynthesis of Chondroitin sulfate (CS), comprising CS by sulfating chondroitin using C4ST or C6ST assisted with a PAPS regeneration system;
- wherein C4ST and C6ST are produced by microorganisms heterogeneously expressing a gene encoding C4ST or C6ST from animals.
Dependencies are inferred from the claim text.