Modified fatty acid biosynthesis with acp-dependent thiolases

47) A genetically engineered microorganism, said microorganism having means for performing a modified fatty acid biosynthesis (FAS) pathway that grows a primer by adding a 2-carbon donor thereto in each cycle, said modified FAS pathway comprising:

• a) an overexpressed acetyl-CoA:ACP transacylase that catalyzes a conversion of acetyl-CoA to an acetyl-ACP primer;
• b) an overexpressed engineered ACP-dependent thiolase that catalyzes a non-decarboxylative condensation of said acyl-ACP primer with a 2-carbon donor acetyl-ACP or acetyl-CoA to produce a ß-ketoacyl-ACP;
• c) an overexpressed 3-oxoacyl-[acyl-carrier-protein] reductase that catalyzes a reduction of said ß-ketoacyl-ACP to a ß-hydroxyacyl-ACP;
• d) an overexpressed 3-hydroxyacyl-[acyl-carrier-protein] dehydratase that catalyzes a dehydration of said ß-hydroxyacyl-ACP to a transenoyl-ACP;
• e) an overexpressed enoyl-[acyl-carrier-protein] reductase that catalyzes a reduction of said transenoyl-ACP to an acyl-ACP(+2) that is two carbons longer than said acyl-ACP primer;
• and,
• f) an overexpressed termination pathway that catalyzes an exit of an intermediate from steps b-e of said modified FAS pathway.

54) The microorganism of claim 5, wherein said microorganism:

• a) expresses a carboxylic acid ω hydroxylase, and produces a product selected from the group consisting of primary alkanolamines (i.e. 1, ω-hydroxyamines), β-hydroxy primary alkanolamines, β-keto primary alkanolamines, and α,β-unsaturated primary alkanolamines;
• b) expresses a carboxylic acid ω hydroxylase, an alcohol oxidase, and an aldehyde dehydrogenase, and produces a product selected from the group consisting of ω-amino acids, β-hydroxy-ω-amino acids, β-keto-ω-amino acids, and α,β-unsaturated ω-amino acids;
• or
• c) expresses a carboxylic acid a hydroxylase, and produces a product selected from the group consisting of α-hydroxylated primary amines, α,β-dihydroxy primary amines, α-hydroxy-β-keto primary amines, and α-hydroxy-α,β-unsaturated primary amines.