Well inserts with brittle membranes
AssigneeSimplinext SA
InventorsSilvia ANGELONI SUTER, Kaspar SUTER, Eric MARGUET, Sylvain BERGERAT, Charlotte VOUTAT
Life of this patent
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Abstract
Disclosed is a well insert for cell culture, including: a membrane support having an upper end and a lower end, the upper end being adapted to engage a well of a microplate so as to suspend the well insert therein; and a permeable membrane for supporting a tissue culture, the permeable membrane being attached at the lower end of the membrane support and sealed thereto, the permeable membrane being of brittle material. The membrane support is overmolded or fastened on to the permeable membrane so as be sealed thereto.
Claims34
1-33. (canceled)
Well insert (10) for cell culture, comprising:
- a membrane support (10a) having an upper end and a lower end, said upper end being adapted to engage a well of a microplate so as to suspend the well insert (10) therein;
- a permeable membrane (1) for supporting a tissue culture, the permeable membrane (1) being attached at said lower end of the membrane support (10a) and sealed thereto, the permeable membrane (1) being of brittle material;
- wherein the membrane support (10a) is made of a polymer exhibiting a linear shrinkage of 1-4% in the radial direction of the permeable membrane (1) which is overmoulded on to the permeable membrane (1) so as be sealed thereto.
Well insert (10) for cell culture, comprising:
- a membrane support (10a) having an upper end and a lower end, said upper end being adapted to engage a well of a microplate so as to suspend the well insert (10) therein;
- a permeable membrane (1) for supporting a tissue culture, the permeable membrane (1) being attached at said lower end of the membrane support (10a) and sealed thereto, the permeable membrane (1) being of brittle material and comprising surface features arranged in a surface thereof;
- wherein the membrane support (10a) is fastened to the permeable membrane (1) in the surface features in the surface thereof.
Well insert (10) for cell culture, comprising:
- a membrane support (10a) comprising an hanger (70) having an upper end and a lower end, said upper end being adapted to engage a well of a microplate so as to suspend the well insert (10) therein;
- a permeable membrane (1) for supporting a tissue culture, the permeable membrane (1) being in arranged at said lower end of the hanger (70) and sealed thereto, the permeable membrane (1) being of brittle material;
- wherein the membrane support (10a) comprises a seal (65) arranged at said lower end of said hanger (70), and wherein said membrane support (10a) further comprises an end piece (60) releasably clipped to said hanger (70) so as to support said permeable membrane (1) between said end piece (60) and said hanger (70) in contact with said seal, said end piece (60) being arranged so as to cause said permeable membrane (1) to compress said seal upon clipping of said end piece on said hanger.
Dependencies are inferred from the claim text.