Recombinant microorganism for improved production of fine chemicals
AssigneeBASF SE
InventorsJennifer JAITZIG, Mukesh KUMAR, Shakir RATANI, Matthew BLANKSCHIEN, Qingzhao WANG
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Abstract
The present invention relates to a recombinant microorganism, to a method for producing alanine and to the use of the recombinant microorganism for the fermentative production of alanine.
Claims30
-30. (canceled)
A recombinant microorganism comprising an introduced, increased or enhanced activity and/or expression of a gcvTHP operon or a homolog or functional variant thereof.
A method for producing a recombinant microorganism with enhanced pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine yield and/or productivity, which comprises the following steps:
- (I) introducing, increasing or enhancing the activity and/or expression of a gcvTHP operon or a homolog or functional variant thereof in a microorganism;
- and
- (II) generating a recombinant microorganism with enhanced pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine yield and/or productivity compared to a reference microorganism without reduced, repressed or deleted activity and/or expression of the gcvTHP operon or a homolog or functional variant thereof.
A recombinant expression construct comprising a promoter functional in a microorganism operably linked to a nucleic acid encoding a gcvTHP operon or a homolog or functional variant thereof.
Dependencies are inferred from the claim text.