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AssigneeBASF SE
InventorsJens SCHRADER, Markus BUCHHAUPT, Frank SONNTAG, Cora KRONER, Heike BRÜSER, Hartwig SCHRÖDER, Ralf PELZER
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The invention relates to microbial terpene production. Known methods for microbial production of terpenes are mostly based on the direct conversion of sugars. Therefore alternative substrates, in particular alternative carbon sources, for use in microbial terpene production were desirable. The invention relates to a methylotrophic bacterium containing recombinant DNA coding for at least one polypeptide with enzymatic activity for heterologous expression in said bacterium, wherein said at least one polypeptide with enzymatic activity is selected from the group consisting an enzyme of a heterologous mevalonate pathway, a heterologous terpene synthase and optionally a heterologous synthase of a prenyl diphosphate precursor. The invention further relates in particular to a method for de novo microbial synthesis of sesquiterpenes or diterpenes from methanol and/or ethanol.
A methylotrophic bacterium containing a heterologous terpene synthase and recombinant DNA coding for at least one polypeptide with enzymatic activity for expression in said bacterium, characterized in that said at least one polypeptide with enzymatic activity is selected from the group consisting of
A methylotrophic bacterium containing a heterologous hydroxymethylglutaryl-CoA synthase (HMG-CoA synthase) and a hydroxymethylglutaryl-CoA reductase (HMG-CoA reductase) as enzymes of a heterologous mevalonate pathway and recombinant DNA coding for at least one polypeptide with enzymatic activity for expression in said bacterium, characterized in that said at least one polypeptide with enzymatic activity is selected from the group consisting of
The bacterium according to any one of 1 to 7, characterized in that the synthase of a prenyl diphosphate precursor is a heterologous FPP synthase, wherein the heterologous FPP synthase is a eukaryotic or prokaryotic FPP synthase.
The bacterium according to any one of 1 to 7, characterized in that the synthase of a prenyl diphosphate precursor is a heterologous GGPP synthase, wherein the heterologous GGPP synthase is an enzyme from an organism which is selected from the group consisting of bacteria, plants and fungi.
Dependencies are inferred from the claim text.