Mutant Yarrowia Strain Capable of Degrading Galactose
AssigneeInstitut National de la Recherche Agronomique
InventorsJean-Marc Nicaud, Zbigniew Lazar, Anne-Marie Crutz-Le Coq, Heber Gamboa-Melen-Dez, Cécile Neuveglise
Life of this patent
Estimating the term…
Abstract
The invention relates to a method for obtaining a mutant Yarrowia yeast strain capable of growing on D-galactose as sole carbon source, comprising over expressing in said strain a galactokinase, a galactose-1-phosphate uridyl transferase, an UDP-glucose-4 epimerase and a galactose mutarotase. The invention also relates to a mutant Yarrowia strain obtained by said method.
Claims16
A method for obtaining a Yarrowia strain capable of growing on D-galactose as sole carbon source, wherein said method comprises overexpressing in said strain a galactokinase (E.C 2.7.1.6) having at least 75% identity with the polypeptide of sequence SEQ ID NO:
- 2, a galactose-1-phosphate uridyl transferase (E.C 2.7.7.12) having at least 65% identity with the polypeptide of sequence SEQ ID NO:
- 4, an UDP-glucose-4 epimerase (E.C5.1.3.2) having at least 85% identity with the polypeptide of sequence SEQ ID NO:
- 6 and a galactose mutarotase (E.C5.1.3.3) having at least 45% identity with the polypeptide of sequence SEQ ID NO:
- 8.
(canceled)
An isolated mutant enzyme selected from the group consisting of:
- a galactokinase (E.C 2.7.1.6) of SEQ ID NO:
- 10, 11, 12 or 13,
- a galactose-1-phosphate uridyl transferase (E.C 2.7.7.12) of SEQ ID NO:
- 15, 16 or 17,
- an UDP-glucose-4 epimerase (E.C 5.1.3.2) of SEQ ID NO:
- 20, 21, 22 or 23, and
- a galactose mutarotase (E.C 5.1.3.3) of SEQ ID NO:
- 27, 28 or 29.
Dependencies are inferred from the claim text.