Binary insecticidal cry toxins
AssigneeDow AgroSciences LLC
InventorsKenneth E. Narva, Huarong Li, Sek Yee Tan, Tao Xu, Timothy D. Hey, Vimbai Chikwana, Sarah E. Worden
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Abstract
The subject invention concerns new classes of insecticidally-active proteins and the polynucleotide sequences which encode these proteins. More specifically, insecticidal proteins of approximately 12-24 kDa and of approximately 12-14 kDa are used for controlling corn rootworms. The subject invention includes methods and transgenic plants for controlling Western Corn Rootworm and other coleopteran insects.
Claims18
A method for controlling a coleopteran pest which comprises exposing the gut of said coleopteran pest to an effective combination of a potentiator protein and a toxin protein.
A nucleic acid construct for expression of a binary toxin comprising a genetic regulatory structure foreign to Bacillus thuringiensis and one or more binary toxin-encoding DNA segments.
A method for producing a coleopteran-tolerant plant comprising breeding a non transgenic plant with a transgenic plant comprising a foreign DNA construct, capable of expressing a binary toxin, stably incorporated into the genome of the coleopteran-tolerant plant and selecting progeny by analyzing for at least a portion of the foreign DNA construct emanating from the transgenic plant.
A composition comprising a formulated binary toxin comprising toxin proteins chosen from the group consisting of:
- a. toxin proteins having at least 70% sequence identity with any one of SEQ ID NOs:
- 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 71, 74, 76, 82, 86, 88, 91, 93, 95, 97, 99, 101, 103, and 105;
- b. toxin proteins having at least 80% sequence identity with any one of SEQ ID NOs:
- 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 71, 74, 76, 82, 86, 88, 91, 93, 95, 97, 99, 101, 103, and 105;
- c. toxin proteins having at least 90% sequence identity with any one of SEQ ID NOs:
- 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 71, 74, 76, 82, 86, 88, 91, 93, 95, 97, 99, 101, 103, and 105;
- d. toxin proteins having at least 95% sequence identity with any one of SEQ ID NOs:
- 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 71, 74, 76, 82, 86, 88, 91, 93, 95, 97, 99, 101, 103, and 105;
- e. toxin proteins having at least 99% sequence identity with any one of SEQ ID NOs:
- 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 71, 74, 76, 82, 86, 88, 91, 93, 95, 97, 99, 101, 103, and 105;
- and,
- f. toxin proteins of SEQ ID NOs:
- 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 71, 74, 76, 82, 86, 88, 91, 93, 95, 97, 99, 101, 103, and 105.
Dependencies are inferred from the claim text.