Cell lines that are free of viral infection and methods for their production
AssigneeBoyce Thompson Institute for Plant Research, Inc.
InventorsRobert Granados, Gary Blissard, Paul Debbie
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Abstract
The present invention relates to cells and cell lines that are free of viral contamination and methods for eliminating viral contamination from a cell or cell line. One exemplary method developed generates Trichoplusia ni cell lines that are free of alphanodavirus. Methods of using a specific, virally-infected cell to generate a virus-free cell are also described herein.
Claims25
A method of making a virus-free cell, the method comprising co-culturing a first cell that is infected with a virus and a second cell that is not infected with the virus, wherein the cells are maintained in culture until the first cell is free of the virus.
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A cell of the cell line having ATCC Accession No. PTA-120815.
A method of making a virus-free cell or reducing the viral load in a cell, the method comprising culturing a first cell that is infected with the virus in a culture medium in which a second cell that is not infected with the virus has been cultured, wherein the culture is maintained for a time sufficient for the first cell to become virus-free or to acquire a reduced viral load.
Dependencies are inferred from the claim text.