In vitro propagation of babesia microti
AssigneeFuller Laboratories
InventorLee Fuller
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Abstract
A method for continuous in vitro propagation of Babesia microti and microti-like species is disclosed. The method comprises incubating B. microti in culture medium comprising host erythrocytes in the presence of a source of complement and a source of anti-B. microti IgM antibody. In one embodiment, the source of anti-B. microti IgM antibody is substantially free of IgG antibody. In one embodiment, the source of complement is a cell or cell line that secretes complement.
Claims29
A method for in vitro propagation of Babesia microti, the method comprising incubating B. microti in culture medium comprising host erythrocytes in the presence of a source of complement and a source of anti-B. microti IgM antibody.
A method for continuous in vitro propagation of B. microti-related hemoparasites, the method comprising incubating the B. microti-related hemoparasites in culture medium comprising host erythrocytes in the presence of a source of complement and a source of anti-B. microti-related hemoparasite IgM antibody.
A propagated culture comprising B. microti or B. microti-related hemoparasites, the propagated culture comprising culture medium comprising host erythrocytes in the presence of a source of complement and a source of anti-B. microti IgM antibody or anti-B. microti-related hemoparasite IgM antibody.
Dependencies are inferred from the claim text.