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US 2018/0073043 A1Published application
Production of Cannabidiolic Acid in Yeast
AssigneeLibrede Inc.
InventorsJason L. Poulos, Anthony N. Farnia
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Abstract
Exemplary embodiments provided herein include genetically engineering microorganisms, such as yeast or bacteria, to produce cannabinoids by inserting genes that produce the appropriate enzymes for the metabolic production of a desired compound.
Claims16
A method for making cannabidiolic acid, the method comprising:
- transforming S. cervisiae with a first nucleotide sequence expressing an acyl-activating enzyme and expressing a mutant prenyltransferase;
- transforming the S. cervisiae with a second nucleotide sequence expressing olivetolic synthase and expressing olivetolic acid cyclase;
- and
- transforming the S. cervisiae with a third nucleotide sequence expressing aromatic prenyltransferase and expressing cannabidiolic acid synthase.
1 more element
A method for making cannabidiolic acid, the method comprising:
- transforming S. cervisiae with a first nucleotide sequence expressing an acyl-activating enzyme;
- transforming the S. cervisiae with a second nucleotide sequence expressing a mutant prenyltransferase;
- transforming the S. cervisiae with a third nucleotide sequence expressing olivetolic synthase;
- transforming the S. cervisiae with a fourth nucleotide sequence expressing olivetolic acid cyclase;
- transforming the S. cervisiae with a fifth nucleotide sequence expressing aromatic prenyltransferase;
- and
- transforming the S. cervisiae with a sixth nucleotide sequence expressing cannabidiolic acid synthase.
4 more elements
A method for making cannabidiolic acid, the method comprising:
- transforming S. cervisiae with a first nucleotide sequence expressing an acyl-activating enzyme and expressing a mutant prenyltransferase;
- transforming the S. cervisiae with a second nucleotide sequence expressing olivetolic synthase and expressing olivetolic acid cyclase;
- and
- transforming the S. cervisiae with a third nucleotide sequence expressing aromatic prenyltransferase and expressing cannabidiolic acid synthase.
1 more element
Dependencies are inferred from the claim text.