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US 2018/0073033 A1Published application
Genes that improve tolerance to lignocellulosic toxins when overexpressed in yeast and methods of use in biofuel production
AssigneeWisconsin Alumni Research Foundation
InventorsMaria Sardi, Audrey P. Gasch
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Abstract
The present invention provides isolated gene sequences useful in increasing lignocellulosic toxin tolerance in yeast. Such engineered yeast are useful in methods of biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast with increased lignocellulosic toxin tolerance are also provided.
Claims18
A recombinant vector comprising:
- (a) the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3;
- or (b) a nucleotide sequence which hybridizes under stringent conditions to SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3 or to a fully complementary nucleotide sequence thereof;
- and (c) a promoter operably-linked to the nucleotide sequence of (a) or (b);
- wherein overexpression in yeast of said nucleotide sequence provides increased tolerance to lignocellulosic toxins relative to a control yeast lacking overexpression of the nucleotide sequence.
1 more element
A method for producing biofuel by fermentation of a lignocellulosic plant material in yeast, comprising:
- (a) culturing under biofuel-producing conditions a recombinant yeast transformed with a recombinant nucleic acid that overexpresses an adenylylsulfate kinase (MET14), protein folding protein folding co-chaperone (MDJ1), or C3 sterol dehydrogenase (ERG26);
- and (b) isolating biofuel produced by said recombinant yeast.
A recombinant Saccharomyces cerevisiae strain, comprising:
- (a) an isolated nucleotide sequence encoding and overexpressing an adenylylsulfate kinase (MET14), protein folding protein folding co-chaperone (MDJ1), or C3 sterol dehydrogenase (ERG26);
- (b) or a nucleotide sequence which hybridizes under stringent conditions to said isolated nucleic acid, or to a fully complementary nucleotide sequence thereof;
- wherein the isolated nucleotide sequence is contained in an extrachromosomal vector maintained at a high copy number in the strain, and said strain exhibits increased tolerance to lignocellulosic toxins relative to a control strain lacking the isolated nucleotide sequence.
Dependencies are inferred from the claim text.