Apobec3a cytidine deaminase induced rna editing
OwnerBora E. BAYSAL, Shraddha SHARMA +1inventors
InventorsBora E. BAYSAL, Shraddha SHARMA, Santosh K. PATNAIK
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Abstract
Provided are methods for identifying agents which can induce or inhibit C>U deamination in RNA driven by apolipoprotein B editing catalytic proteins. The method comprises contacting APOBEC3A or APOBEC3G with a suitable RNA substrate and determining the extent of C>U deamination under conditions which induce APOBEC driven C>U deamination.
Claims12
A method for identifying agents that enhance or inhibit C>U deamination in a RNA molecule comprising:
- a) providing a RNA substrate which contains a motif that contains a C that can undergo deamination to U;
- b) contacting the RNA substrate with a apolipoprotein B editing catalytic (APOBEC) protein in the presence or absence of test agents;
- c) determining the extent of C>U deamination and identifying agents in the presence of which either an increase or decrease of deamination is observed as compared to deamination in the absence of the agent,
- Wherein, the APOBEC protein is APOBEC3A or APOBEC3G.
A method for identifying agents that enhance or inhibit C>U deamination in a RNA substrate comprising:
- a) providing cells which express apolipoprotein B editing catalytic 3A (APOBEC3A);
- b) in the presence or absence of test agents, exposing the cells to conditions under which the cells can carry out APOBEC3A driven C>U deamination of RNA;
- and
- c) determining the extent of C>U deamination in RNA to identify agents that induce or inhibit C>U deamination in RNA,
- wherein an increase in C>U deamination as compared to deamination in the absence of the agent identifies an agent that enhances C>U deamination, and a decrease in C>U deamination as compared to deamination in the absence of the agent identifies an agent that inhibits C>U deamination.
A method for identifying agents that enhance or inhibit C>U deamination in a RNA substrate comprising:
- a) providing cells which have been transfected to overexpress apolipoprotein B editing catalytic3A (APOBEC3A) or apolipoprotein B editing catalytic 3G (APOBEC3G);
- b) in the presence or absence of test agents, determining the extent of C>U deamination in RNA to identify agents that enhance or inhibit C>U deamination in RNA.
Dependencies are inferred from the claim text.