Hydrolases, nucleic acids encoding them and methods for making and using them
AssigneeDsm IP Assets B.V.
InventorsTim Hitchman, Christopher L.G. Dayton, Katie A. Kline, Jonathan Lyon, Mark A. Wall, Nelson R. Barton
Life of this patent
Estimating the term…
Abstract
Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.
Claims17
An isolated, synthetic or recombinant variant polypeptide comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:2, and contains at least one amino acid residue substitution modification relative to SEQ ID NO:2, selected from the group consisting of:
- Y7R, D16M, G27Q, G27S, L43V, G45A, G45L, A48T, S54H, D61A, D61E, D61S, V62E, V62A, V62G, V62M, V62N, V62Q, V62S, V62T, R72E, R72K, R72P, R72S, R72T, R72Y, F74I, F74L, F74P, F74R, G77P, G80P, V83C, V83M, D84V, R89S, A96C, A96I, A96S, G98A, G98L, Y113F, E116A, E116F, E116G, E116H, E116L, E116P, E116Q, E116R, E116S, E116T, E116V, K120I, K120L, K120F, K120M, K120S, K146A, I147F, I147L, I151A, I151G, I151H, I151P, I151S, I151T, T155C, D157S, D157G, D268T, N158A, L159M, P160T, I167R, I167S, R172P, R172Q, R172S, D197K, A210V, A211T, A211S, A211I, S212C, S212A, S212E, S212G, S212H, S212L, S212P, S212Q, S212R, S212T, S212V, S212W, S212Y, K213I, K213G, K213T, T214V, T214P, T214N, T214R, T214Y, G215A, G215H, G215S, G215M, G215V, G215P, G215C, G215W, A216T, A216R, A216Y, A216V, A216C, A216C, A216S, A216L, E217Q, E217R, E217S, E217A, E217G, E217P, A218M, A218H, A218Q, A218R, A218W, A218W, A218S, A218T, A218T, A218K, V223A, V223M, V223R, V223T, V223T, V223F, A224F, A224G, A224I, A224Q, A224Y, A225G, A225L, A225M, A225Q, and A225T, and the variant polypeptide has palmitase activity.
Dependencies are inferred from the claim text.