Theragnostic particles
AssigneeUniversity of Washington through its Center for Commercialization
InventorsCARLOS ENRIQUE CATALANO, JENNY REN-JYE CHANG
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Abstract
The present invention provides viral-based nanoparticles for therapeutic and diagnostic use, and methods for making and using the nanoparticles. Specifically, such nanoparticles comprise decoration-competent viral particles shells such as expanded capsids of phages, stabilized with engineered decoration proteins that have been linked to one or more compounds not naturally occurring on a wild type viral capsid.
Claims21
1-37. (canceled)
A theragnostic particle enveloped by a viral particle shell, wherein:
- at least one engineered decoration protein is bound to the outer surface of the shell, and
- the at least one engineered decoration protein comprises the amino acid sequence of SEQ ID NO:
- 4 (wt gpD), wherein at least one solvent-exposed residue of the at least one engineered decoration protein is mutated to a cysteine or a non-natural amino acid,
- wherein the at least one residue is selected from the group consisting of Ser28, Ala29, Lys30, Asp40, Thr41, Ser42, Ser43, Arg44, Lys45, Gly50, Thr51, Thr52, Asp53, Asp66, Gln67, Thr68, Ser69, Thr70, Thr71, Glu89, Ala90, Ala91, Ser92, Asp93, Glu94, Thr95, Lys96, Lys97, Arg98, and Thr99.
An isolated recombinant protein comprising the amino acid sequence of SEQ ID NO:
- 4 (wt gpD), wherein at least one residue of the protein is mutated to a cysteine or a non-natural amino acid, wherein the at least one residue is selected from the group consisting of Ser28, Ala29, Lys30, Asp40, Thr41, Ser43, Arg44, Lys45, Gly50, Thr51, Thr52, Asp53, Asp66, Gln67, Thr68, Ser69, Thr70, Thr71, Glu89, Ala90, Ala91, Ser92, Asp93, Glu94, Thr95, Lys96, Lys97, Arg98, and Thr99.
Dependencies are inferred from the claim text.